Cell

Part:BBa_J72216

Designed by: Josh Kittleson   Group: Anderson Lab   (2012-09-17)

MC1061 ΔdapD O16::PglpT-rbs1.inv

MC1061 modified by deletion of dapD and replacement of the O16 locus with invasin. Also known as JTK174

Approximate composition of the insertion: BBa_J72163.(rbs1)BBa_J72176.BBa_J27001 - see sequence for exact insertion.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1780
    Illegal XbaI site found at 4498
    Illegal XbaI site found at 4931
    Illegal XbaI site found at 4966
    Illegal PstI site found at 137
    Illegal PstI site found at 4902
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1780
    Illegal NheI site found at 1764
    Illegal PstI site found at 137
    Illegal PstI site found at 4902
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 4887
    Illegal EcoRI site found at 1780
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1780
    Illegal XbaI site found at 4498
    Illegal XbaI site found at 4931
    Illegal XbaI site found at 4966
    Illegal PstI site found at 137
    Illegal PstI site found at 4902
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1780
    Illegal XbaI site found at 4498
    Illegal XbaI site found at 4931
    Illegal XbaI site found at 4966
    Illegal PstI site found at 137
    Illegal PstI site found at 4902
    Illegal NgoMIV site found at 4772
    Illegal AgeI site found at 2684
    Illegal AgeI site found at 4193
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 34


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Categories
Parameters
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